Mutational analysis of two Arabidopsis thaliana cyclin‐dependent kinases in fission yeast

FEBS Letters

De Veyldera, Lieven²  Inzé, Dirk²  Mironov, Vladimir²  Porceddua,, Andrea²  Van Montagu, Marc²  Hayles, Jacky¹ 

[1] ICRF Cell Cycle Laboratory, Lincolns Inn Fields, London WC2A 3PX, UK;Laboratorium voor Genetica, Departement Genetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium

关键词: Cell cycle;    Cyclin-dependent kinase;    CDC2 kinase subunit;    Arabidopsis thaliana;    Schizosaccharomyces pombe;    CDK;    cyclin-dependent kinase;    CKS;    CDC2 kinase subunit;    DAPI;    4′;    6-diamidino-2-phenylindole;   

DOI  :  10.1016/S0014-5793(99)00211-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have analyzed five mutant alleles of two cyclin-dependent kinases from Arabidopsis thaliana, CDC2aAt and CDC2bAt, in Schizosaccharomyces pombe. Two of the five mutant alleles produced similar phenotypes for both cyclin-dependent kinases. The other three mutants caused phenotypes dependent on the particular cyclin-dependent kinase. Of all the mutant alleles, only two were found to possess a detectable kinase activity. Our mutational analysis lends further support for CDC2aAt being the true orthologue of the yeast cdc2. CDC2bAt, even though quite divergent from S. pombe cdc2, still retains the ability to interact with at least some essential cell cycle regulators, suggesting some functional homology with the yeast protein. Additionally, we demonstrated that the three amino acid deletion in the DL50 mutants results in the loss of the ability to interact with the suc1/CKS1 proteins.

【 授权许可】

Unknown