Differential phosphorylation activities of CDK‐activating kinases in Arabidopsis thaliana

FEBS Letters

Umeda, Masaaki¹  Uchimiya, Hirofumi¹  Yamaguchi, Masatoshi¹  Matsubayashi, Satoko¹  Shimotohno, Akie¹ 

[1] Institute of Molecular and Cellular Biosciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-0032, Japan

关键词: Cell cycle;    Cyclin-dependent kinase;    CDK-activating kinase;    Transcription;    Arabidopsis thaliana;    CDK;    cyclin-dependent kinase;    CTD;    carboxy-terminal domain;    CAK;    CDK-activating kinase;    TFIIH;    general transcription factor IIH;    GST;    glutathione S-transferase;    MBP;    maltose-binding protein;   

DOI  :  10.1016/S0014-5793(02)03780-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Activation of cyclin-dependent kinases (CDKs) requires phosphorylation of a threonine residue within the T-loop by a CDK-activating kinase (CAK). Here we isolated an Arabidopsis cDNA (CAK4At) whose predicted product shows a high similarity to vertebrate CDK7/p40^MO15. Northern blot analysis showed that expressions of the four Arabidopsis CAKs (CAK1At–CAK4At) were not dependent on cell division. CAK2At- and CAK4At-immunoprecipitates of Arabidopsis crude extract phosphorylated CDK and the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II with different preferences. These results suggest the existence of differential mechanisms in Arabidopsis that control CDK and CTD phosphorylation by multiple CAKs.

【 授权许可】

Unknown