The Arabidopsis Cks1At protein binds the cyclin‐dependent kinases Cdc2aAt and Cdc2bAt

FEBS Letters

Inzé, Dirk¹  Segers, Gerda¹  De Veylder, Lieven¹  Glab, Nathalie²  Van Montagu, Marc¹  Casteels, Peter¹ 

[1] Laboratorium voor Genetica, Department of Genetics, Flanders Interuniversity Institute for Biotechnology (VIB), Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium;Laboratoire de Physiologie Végétale, Institut de Biotechnologie des Plantes, Université de Paris Sud, F-91405 Orsay-Cedex, France

关键词: Cell cycle;    Cks1At;    Cyclin-dependent kinase;    Yeast two-hybrid screening;    Arabidopsis thaliana;    HB;    homogenization buffer;    MBP;    maltose-binding protein;    NCB;    new column buffer;    PBS;    phosphate-buffered saline;   

DOI  :  10.1016/S0014-5793(97)00822-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In Arabidopsis, two cyclin-dependent kinases (CDK), Cdc2aAt and Cdc2bAt, have been described. Here, we have used the yeast two-hybrid system to identify Arabidopsis proteins interacting with Cdc2aAt. Three different clones were isolated, one of which encodes a Suc1/Cks1 homologue. The functionality of the Arabidopsis Suc1/Cks1 homologue, designed Cks1At, was demonstrated by its ability to rescue the temperature-sensitive cdc2-L7 strain of fission yeast at low and intermediate expression levels. In contrast, high cks1At expression levels inhibited cell division in both mutant and wild-type yeast strains. Cks1At binds both Cdc2aAt and Cdc2bAt in vivo and in vitro. Furthermore, we demonstrate that the fission yeast Suc1 binds Cdc2aAt but only weakly Cdc2bAt, whereas the human CksHs1 associated exclusively with Cdc2aAt.

【 授权许可】

Unknown