Involvement of intact inositol‐1,4,5‐trisphosphate‐sensitive Ca2+ stores in cell cycle progression at the G1/S boundary in serum‐stimulated human fibroblasts

FEBS Letters

Takuwa, Yoh¹  Kumada, Mamoru¹  Takuwa, Noriko¹  Zhou, Wei¹ 

[1] Departments of Cardiovascular Biology and Physiology, Faculty of Medicine, University of Tokyo, Tokyo 113, Japan

关键词: Thapsigargin;    Ca2+ store;    Cell cycle;    Cyclin-dependent kinase;   

DOI  :  10.1016/0014-5793(95)00080-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Thapsigargin, a selective inhibitor of the endoplasmic reticulum Ca²⁺ pump, has been shown to deplete inositol-1,4,5-trisphosphate-sensitive Ca²⁺ stores. Here we report that when thapsigargin was introduced to serum-stimulated human fibroblasts at a time point just before the G1/S boundary, it completely inhibited expression of cyclin A, activation of p33^CDK2 cyclindependent kinase and initiation of DNA synthesis. In contrast, the Ca²⁺ mobilizing ionophore ionomycin was without effect. These findings indicate that Ca²⁺ inside the inositol-1,4,5-trisphosphatesensitive Ca²⁺ stores plays a pivotal role for traverse across the G1/S transition point.

【 授权许可】

Unknown