The cytotoxic effect of Elephantopus scaber Linn extract against breast cancer (T47D) cells

International Pharmacy Conference UAD 2017 on Product Authentication: Key Factor in Quality Control of Pharmaceutical Products

Sulistyani, N.^1 ; Nurkhasanah^1

Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta, Indonesia^1

关键词: Cell cycle;    Cytotoxic;    Elephantopus scaber;    IC50;    T47D;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/259/1/012006/pdf
DOI  :  10.1088/1757-899X/259/1/012006

来源: IOP

【 摘 要 】

Breast cancer is one of the main cause of death. Elephantopus scaber Linn (ES) which has been used as a traditional medicine contains an antitumor compounds. This study aimed to explore the active fraction from ethanolic extract of ES as anticancer and to determine its inhibition effect on the cell proliferation cycle of breast cancer (T47D) cells. The ES leaf was macerated with ethanol and then evaporated to get the concentrated extract. The extract was fractionated using petroleum ether, chloroform, and methanol respectively. The cytotoxic activity of each fraction was carried out with MTT method, and the inhibition of cell cycle test were observed by flowcytometry method. The result showed that ES and the fractions have cytotoxic activity against T47D cell lines with IC50values of extract, petroleum ether, chloroform, and methanol fractions were 58.36±2.38, 132.17±9.69, 7.08±2.11, and 572.89±69.23 g/mL. The inhibition effect of ethanol extract on the lifecycle of cells was occured in sub G1 phase. There was no prolonging of G1, S, G2/M and polyploidy phase of T47D cell lines. The chloroform fraction of ES is the most cytotoxic fraction against T47D cells without prolonging the cell lifecycle.