Phytochemical screening of water extract of gayam (Inocarpus edulis) Bark and its amylase inhibitor activity assay

13th Joint Conference on Chemistry

Fransina, Eirene G.^1 ; Tanasale, Matheis F.J.D.P^1 ; Latupeirissa, Jolantje^1 ; Malle, Dominggus^2 ; Tahapary, Regy^1

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Pattimura University, Ambon, Indonesia^1

Department of Animal Husbandry, Faculty of Agriculture, Pattimura University, Ambon, Indonesia^2

关键词: Alpha-amylase activity;    bark;    Dinitrosalicylic acid method;    gayam (Inocarpus edulis);    IC50;    Inhibitor activity;    Rotary evaporators;    Whatman filter papers;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/509/1/012074/pdf
DOI  :  10.1088/1757-899X/509/1/012074

来源: IOP

【 摘 要 】

The Study on Phytochemical Screening of Water Extract of Gayam (Inocarpus edulis) Bark and its Amylase Inhibitor Activity Assay has been carried out. The aim of this study is to identify the chemical compound of Gayam Bark by phytochemical screening and to determine its amylase inhibitor activity by using dinitrosalicylic acid methods. As much as 15.0212 g of the cut small gayam bark was put into a 250 mL extraction flask. Into the sample was added 100 mL of water and macerated for 24 hours. Then, the mixture was filtered with Whatman filter paper. The filtrate was concentrated using a rotary evaporator to give viscous extract from the Gayam bark. The results showed that the chemical compounds contained in the Gayam bark extract included terpenoids, saponins and tannins. Gayam bark displayed α-amylase enzyme inhibitor where as much as 12.64 μL (17.88 mg/mL) of the extract solution capable of reducing 50% of enzyme α-amylase activity (10 mg/mL). The results showed that the IC50 of extract was 75.3344 μg/mL, which was smaller than that of acarbose, indicating the extract demonstrated better inhibitor activity.