Functional characterization of the diatom cyclin-dependent kinase A2 as a mitotic regulator reveals plant-like properties in a non-green lineage

BMC Plant Biology

Research Article

Lieven De Veylder¹  Marie JJ Huysman²  Chris Bowler³  Atsuko Tanaka⁴  Wim Vyverman⁵ 

[1] Department of Plant Systems Biology, Flanders Institute for Biotechnology (VIB), 9052, Ghent, Belgium;Department of Plant Systems Biology, VIB, and Bioinformatics, Ghent University, 9052, Ghent, Belgium;Department of Plant Systems Biology, Flanders Institute for Biotechnology (VIB), 9052, Ghent, Belgium;Department of Plant Systems Biology, VIB, and Bioinformatics, Ghent University, 9052, Ghent, Belgium;Protistology and Aquatic Ecology, Department of Biology, Ghent University, 9000, Ghent, Belgium;Environmental and Evolutionary Genomics Section, Institut de Biologie de l’Ecole Normale Supérieure, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8186, Institut National de la Santé et de la Recherche Médicale U1024, Ecole Normale Supérieure, 75230, Paris, Cedex 05, France;Environmental and Evolutionary Genomics Section, Institut de Biologie de l’Ecole Normale Supérieure, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8186, Institut National de la Santé et de la Recherche Médicale U1024, Ecole Normale Supérieure, 75230, Paris, Cedex 05, France;Environmental and Evolutionary Genomics Section, Institut de Biologie de l’Ecole Normale Supérieure, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8186, Institut National de la Santé et de la Recherche Médicale U1024, Ecole Normale Supérieure, 75230, Paris, Cedex 05, France;Current address: Muroran Marine Station, Field Science Center for Northern Biosphere, Hokkaido University, 051-0013, Muroran, Hokkaido, Japan;Protistology and Aquatic Ecology, Department of Biology, Ghent University, 9000, Ghent, Belgium;

关键词: Cyclin-dependent kinase;    CDKA2;    Cell cycle;    Cell division;    Cytokinesis;    Diatom;    Mitosis;    Phaeodactylum tricornutum;   

DOI  :  10.1186/s12870-015-0469-6

 received in 2015-01-07, accepted in 2015-02-26,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundCyclin-dependent kinases (CDKs) are crucial regulators of cell cycle progression in eukaryotes. The diatom CDKA2 was originally assigned to the classical A-type CDKs, but its cell cycle phase-specific transcription at the G2-to-M phase transition is typical for plant-specific B-type CDKs.ResultsHere, we report the functional characterization of CDKA2 from the diatom Phaeodactylum tricornutum. Through a yeast two-hybrid library screen, CDKA2 was found to interact with the G2/M-specific CDK scaffolding factor CKS1. Localization of CDKA2 was found to be nuclear in interphase cells, while in cells undergoing cytokinesis, the signal extended to the cell division plane. In addition, overexpression of CDKA2 induced an overall reduction in the cell growth rate. Expression analysis of cell cycle marker genes in the overexpression lines indicates that this growth reduction is primarily due to a prolongation of the mitotic phase.ConclusionsOur study indicates a role for CDKA2 during cell division in diatoms. The functional characterization of a CDK with clear CDKB properties in a non-green organism questions whether the current definition of B-type CDKs being plant-specific might need revision.

【 授权许可】

Unknown   
© Huysman et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.