Levels of endogenous cytokinins, indole‐3‐acetic acid and abscisic acid during the cell cycle of synchronized tobacco BY‐2 cells

FEBS Letters

Inzé, Dirk²  Shaul, Orit²  Redig, Pascale¹  Van Onckelen, Harry¹  Van Montagu, Marc² 

[1] Departement Biologie, Universitaire Instelling Antwerpen, Universiteitsplein 1, B-2610 Antwerpen, Belgium;Laboratorium voor Genetica, Departement Genetica, Flanders Interuniversiteit, Instituut voor Biotechnologie, Universiteit Gent, B-9000 Gent, Belgium

关键词: Cell cycle;    Cytokinin;    Mass spectrometry;    Plant hormone;    Synchronization;    Tobacco BY-2 cell;    ABA;    abscisic acid;    CDK;    cyclin-dependent kinase;    2;    4-D;    2;    4-dichlorophenoxyacetic acid;    DEAE;    diethylaminoethyl;    DZ;    dihydrozeatin;    DZNG;    dihydrozeatin-N-glucoside;    DZMP;    dihydrozeatin riboside-5′-monophosphate;    DZOG;    dihydrozeatin-O-glucoside;    DZROG;    dihydrozeatin riboside-O-glucoside;    DZR;    dihydrozeatin riboside;    GC;    gas chromatography;    gfw;    gram fresh weight;    2iP;    isopentenyladenine;    2iP9G;    isopentenyladenine-9-glucoside;    IAA;    indole-3-acetic acid;    iPMP;    isopentenyladenosine-5′-monophosphate;    iPNG;    isopentenyladenine-N-glucoside;    2iPA;    isopentenyladenosine;    LC;    liquid chromatography;    MAP;    microtubule associating protein;    MS;    mass spectrometry;    RP;    reversed phase;    SIM;    single-ion monitoring;    Z;    zeatin;    Z9G;    zeatine-9-glucoside;    ZMP;    zeatin riboside-5′-monophosphate;    ZNG;    zeatin-N-glucoside;    ZOG;    zeatin-O-glucoside;    ZR;    zeatin riboside;    ZROG;    zeatin riboside-O-glucoside;   

DOI  :  10.1016/0014-5793(96)00728-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Correlation between cell cycle progression and endogenous levels of plant hormones was studied in synchronized tobacco BY-2 cell suspension cultures. Sixteen different cytokinins, indole-3-acetic acid (IAA) and abscisic acid (ABA) were extracted using solid-phase anion exchange chromatography in combination with immunoaffinity purification, and quantified by mass spectrometry. No significant correlation could be identified for IAA and ABA. In contrast, there were sharp peaks in the levels of specific cytokinins (zeatin- and dihydrozeatin-type) at the end of the S phase and during mitosis. The levels of other cytokinins analyzed, including zeatins N- and O-glucosides, remained low, suggesting that the increased amounts of their corresponding non-glucosylated from resulted from de novo synthesis. These findings suggest that zeatin- and dihydrozeatin-type cytokinins might play a specific regulatory role in the progression of the plant cell cycle. One hypothesis to explain cytokinin action is based on a specific interaction with kinases that regulate cell cycle progression, as has been recently shown for the cytokinin analogue olomoucine.

【 授权许可】

Unknown