Characterization of the cytokinin-responsive transcriptome in rice

BMC Plant Biology

Research Article

Ann E. Loraine¹  Ivory C. Blakley¹  G. Eric Schaller²  Jennifer M. Worthen²  Joseph J. Kieber³  Tracy Raines⁴  Yu-Chang Tsai⁵  Roberto Solano⁶  José Manuel Franco-Zorrilla⁷ 

[1] Department of Bioinformatics and Genomics, University of North Carolina at Charlotte, North Carolina Research Campus, 28081, Kannapolis, NC, USA;Department of Biological Sciences, Dartmouth College, 03755, Hanover, NH, USA;Department of Biology, University of North Carolina, 27599-3280, Chapel Hill, NC, USA;Department of Biology, University of North Carolina, 27599-3280, Chapel Hill, NC, USA;Present address: AgBiome, Inc., 104 TW Alexander Drive, Bldg 18, 27713, Research Triangle Park, NC, USA;Department of Biology, University of North Carolina, 27599-3280, Chapel Hill, NC, USA;Present address: Department of Agronomy, National Taiwan University, 10617, Taipei, Taiwan;Department of Plant Molecular Genetics, Centro Nacional de Biotecnología (CNB)-Consejo Superior de Investigaciones Científicas (CSIC), Darwin 3, 28049, Madrid, Spain;Genomics Unit, Centro Nacional de Biotecnología (CNB)-Consejo Superior de Investigaciones Científicas (CSIC), Darwin 3, 28049, Madrid, Spain;

关键词: Rice;    Arabidopsis;    Plant hormone;    Cytokinin;    Transcriptomics;   

DOI  :  10.1186/s12870-016-0932-z

 received in 2016-08-27, accepted in 2016-10-25,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundCytokinin activates transcriptional cascades important for development and the responses to biotic and abiotic stresses. Most of what is known regarding cytokinin-regulated gene expression comes from studies of the dicotyledonous plant Arabidopsis thaliana. To expand the understanding of the cytokinin-regulated transcriptome, we employed RNA-Seq to analyze gene expression in response to cytokinin in roots and shoots of the monocotyledonous plant rice.ResultsWe identified over 4,600 and approximately 2,400 genes differentially expressed in response to cytokinin in roots and shoots respectively. There were some similarities in the sets of cytokinin-regulated genes identified in rice and Arabidopsis, including an up-regulation of genes that act to reduce cytokinin function. Consistent with this, we found that the preferred DNA-binding motif of a rice type-B response regulator is similar to those from Arabidopsis. Analysis of the genes regulated by cytokinin in rice revealed a large number of transcription factors, receptor-like kinases, and genes involved in protein degradation, as well as genes involved in development and the response to biotic stress. Consistent with the over-representation of genes involved in biotic stress, there is a substantial overlap in the genes regulated by cytokinin and those differentially expressed in response to pathogen infection, suggesting that cytokinin plays an integral role in the transcriptional response to pathogens in rice, including the induction of a large number of WRKY transcription factors.ConclusionsThese results begin to unravel the complex gene regulation after cytokinin perception in a crop of agricultural importance and provide insight into the processes and responses modulated by cytokinin in monocots.

【 授权许可】

CC BY   
© The Author(s). 2016