Apoptosis is triggered by the cyclic AMP signalling pathway in renal mesangial cells

FEBS Letters

Sandau, Katrin¹  Nitsch, Dorothea²  Pfeilschifter, Josef²  Varga, Zoltan²  Mühl, Heiko²  Brüne, Bernhard¹ 

[1] Department of Experimental Medicine, University of Erlangen-Nürnberg, D-91054 Erlangen, Germany;Department of Pharmacology, Biozentrum, University of Basel, CH-4056 Basel, Switzerland

关键词: Apoptosis;    Cyclic AMP;    Forskolin;    Salbutamol;    Mesangial cells;   

DOI  :  10.1016/0014-5793(96)00179-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Glomerular mesangial cells are regarded as specialized smooth muscle cells located within the renal glomeruli and fulfilling important functions in glomerular physiology and pathophysiology. Here, we report that activation of the cyclic AMP signalling pathway by dibutyryl cyclic AMP, forskolin, or the β ₂-adrenergic receptor agonist salbutamol results in induction of apoptosis in mesangial cells. Activation of the apoptotic programme results in DNA fragmentation which is visible for most forms of apoptosis and is paralleled by enrichment of cytosolic DNA/histone complexes, an increasing number of cellular 3′-OH-fragmented DNA ends and typical nuclear chromatin condensation. Induction of apoptosis was found to be dependent on translation and independent of nitric oxide synthase activity.

【 授权许可】

Unknown