Cyclic AMP‐elevating agents induce the expression of MAP kinase phosphatase‐1 in PC12 cells

FEBS Letters

Humblot, Nathalie¹  Zwiller, Jean¹  Burgun, Claude¹  Esteve, Laurent¹  Aunis, Dominique¹ 

[1] INSERM U338, Centre de Neurochimie, 5 rue Blaise Pascal, 67084 Strasbourg, France

关键词: Cyclic AMP;    Forskolin;    Immediate early gene;    Mitogen-activated protein kinase phosphatase;    Pituitary adenylyl cyclase-activating polypeptide;    PC12 cell;    MAP;    mitogen-activated protein;    ERK;    extracellular signal-regulated kinase;    TPA;    12-O-tetradecanoyl-phorbol-13-acetate;    JNK;    c-Jun N-terminal kinase;    MKP-1;    MAP kinase phosphatase-1;    PKA;    cAMP-dependent protein kinase;    Bt2cAMP;    dibutyryl cAMP;    Rp-8-Br-cAMPS;    8-bromoadenosine 3′;    5′-monophosphorothioate;    PACAP;    pituitary adenylyl cyclase-activating polypeptide;    BrdU;    bromodeoxyuridine;   

DOI  :  10.1016/S0014-5793(00)02153-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Stimulation of pheochromocytoma PC12 cells by cAMP-elevating agents caused the induction of the immediate early gene 3CH134, which encodes MAP kinase phosphatase-1 (MKP-1). Forskolin was as potent as serum in stimulating MKP-1 gene expression, whereas dibutyryl-cAMP and neuropeptide PACAP were less effective. Induction of the MKP-1 gene was accompanied by neo-synthesis of MKP-1 protein. MAP kinase activation was not involved in the cAMP-induced MKP-1 gene expression. The MAP kinase inactivation, that would result from MKP-1 induction in response to increased intracellular cAMP level, contributes to explain how hormones or neurotransmitters signaling through cAMP influence cell growth and differentiation.

【 授权许可】

Unknown