Forskolin stimulates adenylate cyclase and idodine metabolism in thyroid

FEBS Letters

Cochaux, P.¹  van Sande, J.¹  Dumont, J.E.¹ 

[1] Institut de Recherche Interdisciplinaire, Faculté de Médecine, Université Libre de Bruxelles, Campus Erasme, 808 route de Lennik, B-1000, Brussels, Belgium

关键词: Forskolin;    Dog thyroid;    Cyclic AMP;    Adenylate cyclase;    Iodine metabolism;    TSH;    thyroid stimulating hormone;    PGE1;    prostaglandin E1;    cyclic AMP;    3';    5'-adenosine monophosphate;   

DOI  :  10.1016/0014-5793(82)81321-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Forskolin is a potent activator of the cyclic AMP-generating system in many tissues. In dog thyroid slices, the enhancement of cyclic AMP level was rapid, sustained in the presence of forskolin, but easily reversible after its withdrawal. Contrary to TSH, forskolin induced little apparent desensitization. Forskolin potentiated the effects of TSH, PGE₁ and cholera toxin. However, the forskolin-induced cyclic AMP accumulation was still sensitive to inhibitors of dog thyroid adenylate cyclase such as iodide, norepinephrine and adenosine. As fluoride, but contrary to TSH and PGE₁, forskolin stimulated adenylate cyclase in a medium where Mg²⁺ was replaced by Mn²⁺. This suggests that in thyroid, as in other tissues, forskolin acts beyond the receptor level but, as it potentiates hormone action and does not impair modulation by inhibitors, it may interact with the nucleotide-binding regulatory proteins. Forskolin mimicked the effect of TSH on iodide organification and secretion.

【 授权许可】

Unknown