The existence of a growth‐specific DNA binding factor for the promoter region of mouse ST2 gene

FEBS Letters

Kawakami, Kiyoshi¹  Yanagisawa, Ken²  Kato-Yamazaki, Masako²  Tominaga, Shin-ichi²  Tetsuka, Tsunao² 

[1] Department of Biology, Jichi Medical School, Tochigi, Japan;Department of Biochemistry II, 3311-1 Yakushiji, Minamikawachi-machi, Kawachi-gun, Tochigi 329-04, Japan

关键词: Cell cycle;    Cell growth;    Interleukin 1 receptor;    Immunoglobulin superfamily;    Transcription factor;   

DOI  :  10.1016/0014-5793(94)01152-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A comparison of the 5′-flanking regions of human and mouse ST2 genes revealed the presence of two highly conserved DNA sequences. The promoter activity assay with a luciferase gene as a reporter showed that the deletion of the upstream conserved region diminished the transcriptional activity in growing BALB/c-3T3 cells. By electrophoretic mobility-shift analysis, the presence of a factor that binds to the positive regulatory region of the mouse ST2 gene was found in growing but not in quiescent BALB/c-3T3 cells. These results suggest the functional importance of this conserved region and the requirement of a binding factor for the expression of the ST2 gene.

【 授权许可】

Unknown