Presence of a novel primary response gene ST2L, encoding a product highly similar to the interleukin 1 receptor type 1

FEBS Letters

Tsukamoto, Toshihiko¹  Yanagisawa, Ken¹  Tominaga, Shin-ichi¹  Tetsuka, Tsunao¹  Takagi, Toshimitsu¹ 

[1] Department of Biochemistry II, Jichi Medical School, Tochigi, Japan

关键词: Interleukin 1 receptor;    Cell growth;    Primary response gene;    Immunoglobulin superfamily;    IL-1R1;    interleukin 1 receptor type 1;    dCTP;    deoxy-cytidine 5'-triphosphate;    cDNA;    complementary DNA;    poly(A);    polyadenylic acid;    PCR;    polymerase chain reaction;   

DOI  :  10.1016/0014-5793(93)81333-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the course of studying the ST2 gene, which was initially found to be expressed specifically at the G₀/G₁ transitional state in BALB/C-3T3 cells and was one of the primary response genes, we found another ST2-related mRNA, designated as ST2L, in serum-stimulated BALB/c-3T3 cells in the presence of cycloheximide. Nucleotide sequence analysis of the cloned ST2L cDNA revealed that it had an open reading frame encoding a polypeptide of 567 amino acids. A 5' region (1,028 nucleotides) of ST2L cDNA was identical with the ST2 cDNA, and a unique 3' region encoded a putative transmembrane domain of 24 amino acids and a cytoplasmic domain of 201 amino acids. The ST2 gene product is highly similar to the extracellular portion of IL-1 receptors type 1 and type 2, and the ST2L gene product shows a marked similarity with entire IL-1 receptor type 1.

【 授权许可】

Unknown