Mitogen‐induced expression of the primary response gene cMG1 in a rat intestinal epithelial cell‐line (RIE‐1)

FEBS Letters

Brown, Kenneth D.¹  Gomperts, Miranda¹  Pascall, John C.¹  Corps, Anthony N.¹ 

[1] Department of Biochemistry, AFRC Institute of Animal Physiology and Genetics Research, Babraham Hall, Cambridge, CB24AT, UK

关键词: Primary response gene;    Mitogen stimulation;    Epidermal growth factor;    Angiotensin II;    Protein kinase C;    RIE-1 epithelial cell;    All;    angiotensin II;    EGF;    epidermal growth factor;    FGF;    fibroblast growth factor;    GAPDH;    glyceraldehyde phosphate dehydrogenase;    IBMX;    3-isobutyl-1-methylxanthine;    IGF;    insulin-like growth factor;    PDGF;    platelet-derived growth factor;    TGF-β;    transforming growth factor-β;    TPA;    12-O-tetradecanoyl phorbol-13-acetate;   

DOI  :  10.1016/0014-5793(92)80825-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

cMG1 is a primary response gene first identified in a rat intestinal epithelial (RIE-1)cell-line[(1990) Oncogene 5, 1081–1083]. A number of mitogens, including epidermal growth factor (EGF), angiotensin II (AII), serum and insulin rapidly induced 2- to 6-fold increases of cMG1 mRNA in RIE-1 cells, while transforming growth factor-β caused a small reduction. Cyclic AMP-elevating agents blocked the increase of cMG1 mRNA induced by EGF. The All-stimulated increase of cMG1 mRNA was blocked by the depletion of protein kinase C, whereas the EGF-stimulated increase was not affectcd, indicating that protein kinase C-dependent and -independent signalling pathways stimulate cMG1 expression.

【 授权许可】

Unknown