期刊论文详细信息
FEBS Letters | |
Stabilization of xylanase by random mutagenesis | |
Hata, Yasuo3  Yomo, Tetsuya1  Okada, Hirosuke1  Urabe, Itaru1  Katsube, Yukiteru2  Arase, Akemi1  | |
[1] Department of Biotechnology, Faculty of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565, Japan;Institute for Protein Research, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565, Japan;Institute for Chemical Research, Kyoto University, Gokasho, Uji, Kyoto 611, Japan | |
关键词: Xylanase; Bacillus pumilus; Random mutagenesis; Enzyme stabilization; Heat-resistant mutant; | |
DOI : 10.1016/0014-5793(93)81199-A | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Four heat-resistant mutants of xylanase (N56, N102, N104 and F1) were obtained by random mutagenesis. The mutant genes had the following amino acid changes: N56, Ser-26 to Trp, Gly-38 to Asp and Thr-126 to Ser; N102, Gly-38 to Asp; N104, Gly-38 to Ser and Arg-48 to Lys; F1, Ser-12 to Cys. Kinetic studies showed that N104 is stabilized by an increase in the activation enthalpy, while the other mutants are stabilized by a decrease in the activation entropy.
【 授权许可】
Unknown
【 预 览 】
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