FEBS Letters | |
The complete nucleotide sequence of the xylanase gene (xynA) of Bacillus pumilus | |
Shinmyo, Atsuhiko1  Fukusaki, Eiichiro1  Okada, Hirosuke1  Panbangred, Watanalai1  | |
[1] Department of Fermentation Technology, Osaka University, Yamada-oka Suita-shi, Osaka 565, Japan | |
关键词: Bacillus pumilus; Xylanase; DNA sequence; Signal sequence; Gene cloning; Ribosome binding sequence; | |
DOI : 10.1016/0014-5793(84)80487-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The complete nucleotide sequence of the xylanase (EC 3.2.1.8) gene (xynA and its flanking regions of Bacillus pumilus IPO, a hyperproducer of xylanase, was determined. A 684 bp open reading frame for xylanase gene was observed. The amino acid sequence of the N-terminal region of xylanase was determined to be Arg-Thr-Ile-Thr-, suggesting the processing at Ala27 of pre-xylanase. The amino acid composition and M r (22384) of xylanase deduced from DNA sequence agreed with the results obtained with the purified enzyme. The signal sequence consisted of 27 amino acids, of which 3 were basic amino acid residues in the region near the N-terminus and 15 were hydrophobic amino acid residues. The ribosome binding sequence complementary to the 3′ -end of 16 S rRNA of B, subtilis was found 7 bp upstream of the initiation codon, ATG.
【 授权许可】
Unknown
【 预 览 】
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RO201912020285555ZK.pdf | 381KB | download |