The complete nucleotide sequence of the xylanase gene (xynA) of Bacillus pumilus

FEBS Letters

Shinmyo, Atsuhiko¹  Fukusaki, Eiichiro¹  Okada, Hirosuke¹  Panbangred, Watanalai¹ 

[1] Department of Fermentation Technology, Osaka University, Yamada-oka Suita-shi, Osaka 565, Japan

关键词: Bacillus pumilus;    Xylanase;    DNA sequence;    Signal sequence;    Gene cloning;    Ribosome binding sequence;   

DOI  :  10.1016/0014-5793(84)80487-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The complete nucleotide sequence of the xylanase (EC 3.2.1.8) gene (xynA and its flanking regions of Bacillus pumilus IPO, a hyperproducer of xylanase, was determined. A 684 bp open reading frame for xylanase gene was observed. The amino acid sequence of the N-terminal region of xylanase was determined to be Arg-Thr-Ile-Thr-, suggesting the processing at Ala²⁷ of pre-xylanase. The amino acid composition and M _r (22384) of xylanase deduced from DNA sequence agreed with the results obtained with the purified enzyme. The signal sequence consisted of 27 amino acids, of which 3 were basic amino acid residues in the region near the N-terminus and 15 were hydrophobic amino acid residues. The ribosome binding sequence complementary to the 3′ -end of 16 S rRNA of B, subtilis was found 7 bp upstream of the initiation codon, ATG.

【 授权许可】

Unknown