Statistical analysis and modeling of nucleosome positioning

Nucleosome;Nucleosome positioning;Nucleosome occupancy;DNA sequence;Statistical mechanics;Chromatin;Cross-entropy;10-bp periodicity, ,

Jin, Hu

关键词: Nucleosome;    Nucleosome positioning;    Nucleosome occupancy;    DNA sequence;    Statistical mechanics;    Chromatin;    Cross-entropy;    10-bp periodicity, ,;   

Others  :  https://www.ideals.illinois.edu/bitstream/handle/2142/100991/JIN-DISSERTATION-2018.pdf?sequence=1&isAllowed=y

美国|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

Nucleosome is the fundamental building block of eukaryotic chromatin. The precise location of nucleosome along the genome plays a central role in gene regulation by controlling the accessibility of genetic information encoded in DNA. Understanding the principles underlying the establishment of genome-wide nucleosome landscape is a critical step towards understanding chromatin structure and diverse cellular processes taking place on the chromatin template. Recent studies have identified various factors that impact the nucleosome landscape in vivo, such as DNA sequence, statistical positioning, and chromatin remodelers; but the results remain largely unconnected and sometimes even provide conflicting views. A comprehensive study integrating different determinants of nucleosome landscape into one consistent framework is still missing. Taking advantage of the flourishing experimental data generated by high-throughput sequencing technology, this thesis applies novel statistical analysis methods and statistical mechanics modeling to the nucleosome positioning problem. We start from quantifying the extent of DNA-encoded sequence signatures and characterizing their respective contributions to different aspects of nucleosome landscape. We then integrate multiple determinants of nucleosome landscape into a unified computational framework and investigate how different factors work together to accurately regulate nucleosome organization.