Isolation and Identification of cellulolytic bacteria from mangrove sediment in Bangka Island

Asean-Fen International Fisheries Symposium - 2017

Kurniawan, A.^1,2 ; Prihanto, A.A.^3 ; Sari, S.P.^4 ; Febriyanti, D.^1 ; Kurniawan, A.^5 ; Sambah, A.B.^6 ; Asriani, E.^7

Departement of Aquaculture, Bangka Belitung University, Balunijuk, Merawang, Bangka City, Indonesia^1

Graduate School of Fisheries Science, Faculty of Fisheries and Marine Science, Brawijaya University, Jl. Veteran, East Java, Malang

65145, Indonesia^2

Departement of Fishery Product Technology, Faculty of Fisheries and Marine Science, Brawijaya University, Jl. Veteran, East Java, Malang

65145, Indonesia^3

Departement of Marine Science, Bangka Belitung University, Balunijuk, Merawang, Bangka City, Indonesia^4

Departement of Aquatic Resource Management, Faculty of Fisheries and Marine Science, Brawijaya University, Jl. Veteran, East Java, Malang

65145, Indonesia^5

Departement of Utilization of Fisheries Reources, Faculty of Fisheries and Marine Science, Brawijaya University, Jl. Veteran, East Java, Malang

65145, Indonesia^6

Departement of Agrotechnology, Bangka Belitung University, Balunijuk, Merawang, Bangka City, Indonesia^7

关键词: Bacillus pumilus;    Cellulolytic bacterium;    Hydrolyze cellulose;    Isolation and identification;    Mangrove sediments;    Pseudomonas aeruginosa;    Pseudomonas sp;    Reducing sugars;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/137/1/012070/pdf
DOI  :  10.1088/1755-1315/137/1/012070

来源: IOP

【 摘 要 】

Cellulolytic bacteria is bacteria which hydrolyze cellulose to reducing sugars. This research aims to obtain cellulolytic bacteria from the sediment of mangroves in Bangka island. Reasearch was conducted from March to August 2017. Sampling was conducted at Sungailiat, and Tukak Sadai, South of Bangka. Bacteria was isolated using 1% Carboxymetyl Cellulosa (CMC). The isolation resulted in four isolates from Sungailiat and nine isolates from Tukak Sadai. Total five isolates, namely Bacillus pumilus, Pseudomonas sp., Bacillus amyloliquefacien, Bacillus alvei, Bacillus coagulant were identified. The best isolates that produced cellulose was Pseudomonas aeruginosa.