Characterization of phytase enzymes as feed additive for poultry and feed

Asean-Fen International Fisheries Symposium - 2017

Lamid, M.^1,2,3 ; Al-Arif, A.^1 ; Asmarani, O.^3 ; Warsito, S.H.^1,4

Faculty of Veterinary Medicine, Airlangga University, Surabaya

60115, Indonesia^1

Faculty of Fisheries and Marine, Airlangga University, Surabaya

60115, Indonesia^2

Proteomics Laboratory, Institute of Tropical Disease, Airlangga University, Surabaya

60115, Indonesia^3

Faculty of Vocational, Airlangga University, Surabaya

60115, Indonesia^4

关键词: Actinobacillus sp;    Bacillus pumilus;    Complex compounds;    Mineral availability;    Optimum temperature;    Phytase activity;    Phytase enzymes;    UV-Vis spectrophotometers;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/137/1/012009/pdf
DOI  :  10.1088/1755-1315/137/1/012009

来源: IOP

【 摘 要 】

One of the obstacles to utilizing rice bran as feed is the presence of antinutrition in the form of phytic acid which binds in minerals to form complex compounds with P, Mg, Mn, Fe, Zn, Ca. Phytic acid and its salts are the main forms of P, Mg, Mn, Fe, Zn, Ca deposits contained in cereals, legume and grains, about 60-90% of total minerals P, Mg, Mn, Fe, Zn, Ca in the form of phytic acid or phytate salts. Phytate is one of the enzymes belonging to the phosphatase group capable of hydrolyzing phytate compounds of myo-inositol (1,2,3,4,5,6) hexsa phosphatase into myo-inositol and organic phosphat. The aim of this study was to obtain characterization of phytase enzymes from isolate Actinobacillus sp., Bacillus pumilus, Bacillus vallimortis and IBR-1. Determination of phytase activity and the absorbance was measured using a UV-Vis spectrophotometer at a wavelength of 392 nm. The result of Actinobacillus sp, Bacillus pumilus, Bacillus vallimortis, IBR-1 each having optimum temperature were 50°C, 40°C, 45°C, 45°C, and optimum pH were 4, 4, 5.5. Bacteria especially Actinobacillus sp, Bacillus pumilus, Bacillus vallimortis, IBR-1 are proven capable of producing the high enough phytase enzymes required for mineral availability for livestock and fish.