Molecular characterization of a proteolysis-resistant lipase from Bacillus pumilus SG2

Revista de microbiologia

Arulpandi, I.¹  Geetha, A.²  Sangeetha, R.³ 

[1] Asan Memorial College, Chennai, India;Bharathi Women's College, Chennai, India;Vels University, Chennai, India

关键词: Bacillus pumilus;    covalent dimer;    lipase;    proteolytic-resistance.;   

DOI  :  10.1590/S1517-83822014000200004

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

Proteolysis-resistant lipases can be well exploited by industrial processes which employ both lipase and protease as biocatalysts. A proteolysis resistant lipase from Bacillus pumilus SG2 was isolated, purified and characterized earlier. The lipase was resistant to native and commercial proteases. In the present work, we have characterized the lip gene which encodes the proteolysis-resistant lipase from Bacillus pumilus SG2. The parameters and structural details of lipase were analysed. The lip gene consisted of 650 bp. The experimental molecular weight of SG2 lipase was nearly double that of its theoretical molecular weight, thus suggesting the existence of the functional lipase as a covalent dimer. The proteolytic cleavage sites of the lipase would have been made inaccessible by dimerisation, thus rendering the lipase resistant to protease.

【 授权许可】

CC BY-NC