Proteomics study of extracellular fibrinolytic proteases from Bacillus licheniformis RO3 and Bacillus pumilus 2.g isolated from Indonesian fermented food

2nd International Conference on Tropical and Coastal Region Eco Development 2016

地球科学;生态环境科学

Afifah, Diana Nur^1 ; Rustanti, Ninik^1 ; Anjani, Gemala^1 ; Syah, Dahrul^2 ; Yanti^3 ; Suhartono, Maggy T.^2

Department of Nutrition Science, Faculty of Medicine, Diponegoro University, Indonesia^1

Department of Food Science and Technology, Faculty of Agricultural Technology, Bogor Agricultural University, Indonesia^2

Faculty of Biotechnology, Atma Jaya Catholic University, Indonesia^3

关键词: Bacillus licheniformis;    Bacillus pumilus;    Extracellular;    Fermented foods;    Mass spectrometry analysis;    Protein database;    Protein fractions;    Two dimensional electrophoresis;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/55/1/012025/pdf
DOI  :  10.1088/1755-1315/55/1/012025

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

This paper presents the proteomics study which includes separation, identification and characterization of proteins. The experiment on Indonesian fermented food such as extracellular fibrinolytic protease from Bacillus licheniformis RO3 and Bacillus pumilus 2.g isolated from red oncom and tempeh gembus was conducted. The experimental works comprise the following steps: (1) a combination of one- and two-dimensional electrophoresis analysis, (2) mass spectrometry analysis using MALDI-TOF-MS and (3) investigation using protein database. The result suggested that there were new two protein fractions of B. licheniformis RO3 and three protein fractions of B. pumilus 2.g. These result has not been previously reported.