Electrical potential dissipation induced by free fatty acids in pea stem mitochondria

FEBS Letters

Petrussa, E.¹  Vianello, A.¹  Macri, F.¹  Nagy, G.¹  Braidot, E.¹ 

[1] Section of Plant Physiology and Biochemistry, Institute of Plant Protection, University, of Udine, via Cotonificio 108, I-33100 Udine, Italy

关键词: Fatty acid;    Mitochondrion;    ATP/ADP antiporter;    Lipid peroxidation;    Pisum sativum L;    BHT;    butylated hydroxytoluene;    CAtr;    carboxyatractyloside;    FFA;    free fatty acid(s);    FCCP;    carbonyl cyanide p-(triphluoromethoxy)phenylhydrazone;    MDA;    malondialdehyde;    TBA;    thiobarbituric acid;   

DOI  :  10.1016/0014-5793(92)80692-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Linolenic, linoleic, oleic, palmitic and stearic acids (FFA) collapse the electrical potential of pea stem mitochondria in the absence or in the presence of 0.5 mM Mg²⁺, Higher concentrations of this cation (5 mM) lower the rate or dissipation caused by linoleic, oleic and palmitic acids, while abolishing that induced by stearic acid. Carboxyatractyloside and ADP do not reverse the FFA-induced collapse both in the presence or absence of Mg²⁺. EDTA, EGTA or BHT do not influence the dissipation caused by FFA that, in addition, is not linked to lipid peroxidation evaluated as malondialdehyde or conjugated diene formation. Only linolenic acid sustains a peroxidation which, however, appears to be caused by its owm oxidation catalysed by lipoxygenases rather than by membrane lipoperoxidation induced by this free fatty acid. These results suggest that neither the ATP/ADP exchanger nor lipid peroxidation appear to be involved in FFA-induced uncoupling in pea stem mitochondria.

【 授权许可】

Unknown