Use of waste from the leather industry for the production of biotechnological products based on collagen

International Conference Baltic Polymer Symposium 2018

Savchuk, O.M.^1^2 ; Rebrikova, P.A.^1 ; Vovk, T.B.^2 ; Raksha, N.G.^2 ; Ostapchenko, L.I.^2 ; Mokrousova, O.R.^1

Department of Biotechnology, Leather and fur, Kiev National University of Technology and Design, Nemirovicha-Danchenko str., 2, Kyiv

01011, Ukraine^1

Department of Biochemistry, Educational and Scientific Center, Institute of Biology and Medicine, Taras Shevchenko National University of Kyiv, 64/13, Volodymyrska str., Kyiv

01601, Ukraine^2

关键词: Anti-inflammatories;    Antioxidant enzyme;    Collagen peptides;    Leather industries;    Lipid peroxidation;    Pharmaceutical industry;    Pro-inflammatory cytokines;    Sodium dodecyl sulphate;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/500/1/012020/pdf
DOI  :  10.1088/1757-899X/500/1/012020

来源: IOP

【 摘 要 】

Leather industry generates vast quantities of by-products which could be a cheap and available source of collagen and collagen peptides. These peptides have been widely used in cosmetic, biomedical, and pharmaceutical industries. The main objective of present study was to obtain a biotechnological product based on collagen and to study its properties. Lime fleshings from hides were fragmented into small pieces with size ≤ 0.5 cm2. Collagen was extracted from trimming waste using 0.5 M acetic acid in the presence of 5 mM EDTA. The purity of extracted collagen was checked by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Collagen was enzymatically hydrolyzed by pepsin. To stop the reaction, the mixture was heated immediately at 100°C and then centrifuged at 4000 g for 30 min. The supernatant was filtered through ceramic membrane for separation of collagen peptides fraction (26≤kDa). Collagen peptides were prepared from the obtained collagen. The generated collagen peptides was analyzed by SDS-PAGE using 18% resolving gel. The influence of collagen peptides on antioxidant enzymes and lipid peroxidation were explored. Collagen peptides supplementation also lowered serum level of pro-inflammatory cytokines such as IL-1β, IL-12, while level of anti-inflammatory cytokine IL-4 was within the control value.