Carboxyatractylate inhibits the uncoupling effect of free fatty acids

FEBS Letters

Volkov, N.I.¹  Bondareva, T.O.¹  Dedukhova, V.I.¹  Andreyev, A.Yu.¹  Skulachev, V.P.¹  Mokhova, E.N.¹ 

[1]A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Moscow 119899 USSR

关键词: Fatty acid;    Uncoupler;    Mitochondria;    ATP/ADP antiporter;    Carboxyatractylate;    BLM;    bilayer phospholipid membrane;    BSA;    bovine serum albumin;    CAtr;    carboxyatractylate;    DNP;    2;    4-dinitrophenol;    BHT;    2;    6-di(t-butyl)-p-cresol;    FCCP;    p-trifluoromethoxyphenylhydrazonecarbonyl cyanide;    Mops;    morpholinopropane sulphonate;   

DOI  :  10.1016/0014-5793(88)81436-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The ATP/ADP-antiporter inhibitors and ADP decrease the palmitate-induced stimulation of the mitochondrial respiration in the controlled state. The degree of inhibition decreases in the order: carboxyatractylate > bongkrekic acid, palmitoyl-CoA, ADP > atractylate. GDP is ineffective. The inhibiting concentration of carboxyatractylate coincides with this arresting the state 3 respiration. Carboxyatractylate inhibition decreases when the palmitate concentration increases. Stimulation of controlled respiration by FCCP or gramicidin D at any concentration of these uncouplers is carboxyatractylate-resistant, whereas that by low concentrations of DNP is partially suppressed by carboxyatractylate. These data together with observations that palmitate does not increase H⁺ conductance in bilayer phospholipid membranes and in cytochrome oxidase-asolectin proteoliposomes indicate that the ATP/ADP-antiporter is somehow involved in the uncoupling by low concentrations of fatty acids (or DNP), whereas that by FCCP and gramicidin D is due to their effect on the phospholipid bilayer. It is suggested that the antiporter facilitates translocation of palmitate anion across the mitochondrial membrane.

【 授权许可】

Unknown