Free fatty acids decouple oxidative phosphorylation by dissipating intramembranal protons without inhibiting ATP synthesis driven by the proton electrochemical gradient

FEBS Letters

Rottenberg, Hagai¹  Steiner-Mordoch, Sonia¹ 

[1] Pathology Department, Hahnemann University School of Medicine, Broad and Vine, Philadelphia, PA 19102, USA

关键词: Oxidative phosphorylation;    Mitochondria;    Uncoupler;    Fatty acid;    Chemiosmosis;    Proton electrochemical difference;   

DOI  :  10.1016/0014-5793(86)80708-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Free fatty acids (FFA) uncouple oxidative phosphorylation and reverse electron transport and inhibit ATP-P_i exchange in beef heart submitochondrial particles. In this, they resemble classical uncouplers and ionophores. However, in contrast to the latter agents, FFA do not collapse the substrate generated proton electrochemical potential and do not inhibit ATP synthesis when the latter is driven by artificially imposed H. These results lend further support to the suggestion that oxidative phosphorylation depends, in part, on direct intramembranal proton transfer - a process which is specifically uncoupled by FFA and other membrane perturbing agents (e.g. general anesthetics).

【 授权许可】

Unknown