The Saccharomyces cerevisiae CDC25 gene product is a 180 kDa polypeptide and is associated with a membrane fraction

FEBS Letters

Jacquet, M.¹  Garreau, H.²  Camonis, J.H.²  Guitton, C.¹ 

[1] Institut Pasteur, Département de Biochimie Cellulaire, 25 rue du Docteur Roux, 75724 Paris cedex 15, France;Groupe IGD, Institut de Génétique et de Microbiologie, URA CNRS 1354 bât, 400, Université Paris XI, 91405 Orsay, France

关键词: Cell cycle;    Signal transduction;    RAS;    CAMP pathway;   

DOI  :  10.1016/0014-5793(90)81117-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the yeast Saccharomyces cerevisiae, the CDC25 gene product is supposed to interact with ras proteins and adenylate cyclase for progression through the cell division cycle. To identify the CDC25 gene product, we raised antibodies against two hybrid proteins, encoded by in-frame fusions between the E. coli lacZ gene and two different parts of the CDC25 gene. By protein immuno-blotting, we were able to identify the CDC25 gene product as a 180 kDa polypeptide, which we named p180^CDC25. It was detected only when the CDC25 gene was overexpressed in a proteases-deficient yeast strain. Subcellular fractionation experiments showed that p180^CDC25, as well as ras proteins, is attached to the membrane, even after treatments which release peripheral membrane proteins.

【 授权许可】

Unknown