Stathmin overexpression in 293 cells affects signal transduction and cell growth

FEBS Letters

Gavet, Olivier¹  Lawler, Sean¹  Rich, Tina²  Sobel, André¹ 

[1] INSERM U440, 17 Rue du Fer à Moulin, 75005 Paris, France;INSERM U396, Laboratoire Immunogénétique Humaine, Institut Biomédical de Cordeliers, 75005 Paris, France

关键词: Stathmin;    Signal transduction;    Cell cycle;    Protein phosphorylation;   

DOI  :  10.1016/S0014-5793(97)01519-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Stathmin is a ubiquitous cytoplasmic protein whose phosphorylation state changes markedly in response to extracellular signals, and during the cell cycle. To clarify the function of stathmin, its four phosphorylation sites were mutated to either alanines (4A-stathmin) or glutamates (4E-stathmin). In transfected cells, 4A-stathmin caused a strong G2/M block and also inhibited the responsiveness of a co-transfected fos promoter/luciferase reporter plasmid to serum stimulation, whereas wild type and 4E-stathmin had relatively minor effects. These results support the idea that stathmin plays a role in multiple cellular processes and indicate that the regulation of the phosphorylation state of stathmin is likely to determine its action.

【 授权许可】

Unknown