Stathmin phosphorylation patterns discriminate between distinct transduction pathways of human T lymphocyte activation through CD2 triggering

FEBS Letters

Chneiweiss, Herve²  Debre, Patrice¹  Tarantino, Nadine¹  le Gouvello, Sabine¹  Sobel, Andre² 

[1] CNRS URA 625, C.E.R. V.I., C.H. Pitié-Salpètrière, 83 Bd de l'Hopital. 75013 Paris, France;INSERM U 153 - CNRS U'RA 614, 17 rue du Fer à Moulin, 75005 Paris, France

关键词: Stathmin;    T lymphocyte activation;    CD2;    Protein kinase C;    Protein phosphorylation;    DAG;    diacylglycerol;    2D-PAGE;    two-dimensional polyacrylamide gel electrophoresis;    PS;    phosphatidylserine;    PKC;    protein kinase C;    TPA;    12-O-tetradecanoylphorbol-13-acetate;   

DOI  :  10.1016/0014-5793(91)80020-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

CD2 triggering of human T lymphocyte activation has been associated with the activation of different interacting protein kinases, including protein kinase C (PKC). However the precise roles of its phosphorylated substrates are still unknown. We show here that PKC-dependent and -independent pathways arc responsible for the CD2-induced phosphorylation of stathmin, a ubiquitous soluble phosphoprotein, most likely acting as a general intracellular relay integrating various second messenger pathways. The phosphorylated variants of stathmin provide a fingerprint reflecting the second messenger pathway(s) stimulated. The respective roles of both PKC and stathmin in the regulation of T lymphocyte proliferation are discussed.

【 授权许可】

Unknown