Secretion of endothelin and related peptides from renal epithelial cell lines

FEBS Letters

Marumo, F.²  Sato, K.¹  Nakajima, T.¹  Emori, T.²  Shichiri, M.²  Sato, A.¹  Hirata, Y.²  Ohta, K.² 

[1] Department of Biomaterials Science, Tokyo Medical and Dental University, Tokyo 113, Japan;Department of Internal Medicine, Tokyo Medical and Dental University, Tokyo 113, Japan

关键词: Endothelin;    HPLC;    reverse-phase;    RIA;    (Renal cell line);   

DOI  :  10.1016/0014-5793(89)80959-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using specific radioimmunoassays (RIAs) for endothelin (ET) and big ET, we have studied whether ET and related peptides are secreted from renal epithelial cell lines (LLCPK₁ and MDCK) of non-endothelial origin. Dilution curves of extracts of conditioned media from both LLCPK₁ and MDCK cell lines were parallel to those of standard porcine (p) ET and big pET in each RIA. Both cell lines incubated in serum-free medium secreted ET- and C-terminal fragment (CTF)-like immunoreactivity (LI) of big ET as a function of time. Reverse-phase HPLC coupled with both RIAs of the extracted media from both cell lines revealed a single component with ET-LI coeluting with pET(1–21) and several components with CTF-LI, one corresponding to the elution position of big pET(1–39), one to its CTF(22–39), and the others eluting earlier than CTF. These data indicate that endothelin and related peptides are synthesized by and secreted from cells other than endothelial cells.

【 授权许可】

Unknown