Determination of Quercetin and Rutin in Red Galangal Rhizomes (Alpinia purpurata) and White Galangal (Alpinia galanga) with High Performance Liquid Chromatography Method

International Conference on Food Science and Technology

Suzery, M.^1 ; Ningrum, A.N.^1 ; Nudin, B.^1 ; Mulyani, N.S.^1 ; Cahyono, B.^1

Department of Chemistry, Faculty of Science and Mathematics, Diponegoro University, Semarang, Indonesia^1

关键词: Alpinia galanga;    Alpinia purpurata;    HPLC;    Quercetin;    Rutin;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/292/1/012064/pdf
DOI  :  10.1088/1755-1315/292/1/012064

来源: IOP

【 摘 要 】

Research of flavonoid determination (quercetin and rutin) in Alpinia purpurata and Alpinia galanga rhizomes using HPLC method has been conducted. This study aims to determine the qualitative and quantitative presence of rutin and quercetin compounds in Alpinia purpurata and Alpinia galanga rhizomes. Fractions of ethyl acetate and n-butanol have been obtained by TLC, followed by total flavonoid analysis with UV-Vis spectrophotometer method and quercetin and rutin determination with HPLC. The results obtained in this study are as follows: Total flavonoid from ethyl acetate fraction obtained is 46,48 mg EQ/gram in Alpinia purpurata and 70,60 mg EQ/gram in Alpinia galanga, and total flavonoid from nbutanol fraction obtained is 68,50 mg EQ/gram for Alpinia purpurata and 103,80 mg EQ/gram for Alpinia galanga. The ethyl acetate fraction of Alpinia purpurata detected quercetin content at 5469.64 mg/kg and rutin at 4955.59 mg/kg, while Alpinia galanga detected quercetin at 5764.10 mg/kg and rutin at 5327.93 mg/kg. From n-butanol fraction, quercetin are only detected at 6737.14 mg/kg in Alpini purpurata and 9098.74 mg/kg in Alpinia galanga. From these results, it can be concluded that HPLC method can be used to detect the presence of flavonoid quercetin and rutin in Alpinia purpurata and Alpinia galanga rhizomes.