Alkaloids piperine in dichloromethane fraction of red galangal rizhome (Alpinia purpurata)

13th Joint Conference on Chemistry

Suzery, Meiny^1 ; Ria, Resti Yuyun Septembe^1 ; Cahyono, Bambang^1

Department of Chemistry, Faculty of Science and Mathematics, Diponegoro University, Semarang

50267, Indonesia^1

关键词: Alpinia purpurata;    Bioactive compounds;    Chemical formulae;    NMR spectrometer;    Piperine;    red galangal rizhome;    Spectroscopic method;    UV-vis spectrometer;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/509/1/012076/pdf
DOI  :  10.1088/1757-899X/509/1/012076

来源: IOP

【 摘 要 】

Red galangal rizhome (Alpiniapurpurata) was extracted with dichloromethane using maceration methods. The crude extract was fractionated using vacuum column chromatography with a mixture of ethyl acetate:n-hexane (2:3) to give two fractions (F1 and F2). Fraction 2 was fed into the silica gel of column chromatography tailed by recrystallization to produce crystalline (17 mg). The purity was tested by using melting point apparatus and thin-layer chromatography method for any solvent. Their structures were established by spectroscopic methods (UV-Vis, FTIR, 1H-NMR and 13C-NMR). Result of analysis gave melting point of crystals around 130°C. The UV-Vis spectrometer obtained maximum wavelength 344 nm. FTIR analysis showed C=O carbonyl, C-O ether, C-N, and C=C aromatic. Analysis by 1H-NMR spectrometer showed that bioactive compounds have a 19 protons. Analysis by 13C-NMR spectrometer showed that bioactive compounds have a 17 carbon atoms. The results of identification showed that bioactive compounds in dichloromethane fraction of red galangal rhizome was a compound piperine alkaloids types with a chemical formula of C17H19O3N.