Autocrine receptors for endothelins in the primary culture of endothelial cells of human umbilical vein

FEBS Letters

Fujitani, Yasushi¹  Inui, Takashi¹  Okada, Toshikazu¹  Urade, Yoshihiro¹  Oda, Kyoko¹  Takimoto, Misato¹ 

[1] International Research Laboratories, CIBA-GEIGY (Japan) Limited, 10-66 Miyuki-cho, Takarazuka 665, Japan

关键词: Endothelin;    Endothelial cell;    Receptor;    Vasodilation;    EC;    endothelial cell;    ET;    endothelin;    HBSS;    HEPES-buffered salt solution;    HEPES;    N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid;    HUVECs;    human umbilical vein endothelial cells;    RIA;    Radioimmunoassay;   

DOI  :  10.1016/0014-5793(92)80026-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human umbilical vein endothelial cells (HUVECs) in primary culture produced and secreted endothelin I (ET-1) actively. Specific binding of [¹²⁵I]ET-1 to these cells was not detectable because of the saturation of ET receptors with endogenously produced ET-1. However, addition of phosphoramidon, an inhibitor of ET-converting enzyme, to the medium reduced the production of ET-1 and thus the receptors on HUVECs were made available for exogenously added [¹²⁵I]ET-1. Binding studies using phosphoramidon-treated HUVECs indicated the existence of a non-isopeptide-selective type (ET_B) or ET receptor with a K _d of 17 pM. This receptor is thought to be involved in ET-induced vasodilation in autocrine manner in vivo.

【 授权许可】

Unknown