Presence of non‐selective type of endothelin receptor on vascular endothelium and its linkage to vasodilation

FEBS Letters

Ohnaka, Keizo¹  Nawata, Hajime¹  Aimoto, Saburo⁴  Kitazumi, Kazuhiro²  Tasaka, Kenjí²  Ohashi, Masao¹  Takasaki, Chikahisa³  Takayanagi, Ryoichi¹ 

[1] The Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka 812, Japan;Department of Pharmacology, Faculty of Pharmaceutical Sciences, Okayama University, Okayama 700, Japan;Department of Chemistry, Faculty of Science, Tohaku University, Sendai 980, Japan;Institute for Protein Research, Osaka University, Osaka 565, Japan

关键词: Endothelin;    Sarafotoxin;    Endothelium;    Endothelial cell;    Receptor;    Vasodilation;   

DOI  :  10.1016/0014-5793(91)80454-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We studied the role of non-selective type (ET₁₄) of endothelin (ET) receptor in the vasculature, using a ligand specific to the ET₁₄ receptor, [Glu⁹]-sarafotoxin S6b ([Glu⁹]SRTb). Endothelium-containing rat thoracic aorta possessed specific binding sites for ¹²⁵I-[Glu⁹]SRTb, which were almost eliminated by removal of the endothelium, while ET-3-specific binding sites were not detected in the endothelium-intact rat aorta. Only ET₁₄ receptor was detected in the membranes from the endothelium of porcine thoracic aorta. [Glu⁹]SRTb exerted only vasodilation in rat aortic ring. These findings indicate that ET₁₄ receptors are located on vascular endothelium and linked to vasodilation.

【 授权许可】

Unknown