Brain natriuretic peptide‐like immunoreactivity is present in human plasma

FEBS Letters

Togashi, Kazuyoshi²  Hirata, Yukio¹  Kawakami, Masaya⁴  Marumo, Fumiaki¹  Takei, Yoshio³  Ando, Kenji² 

[1] The Second Department of Internal Medicine, Tokyo Medical and Dental University, Tokyo 113, Japan;Kitasato Biochemical Laboratories, Kitasato University School of Medicine, Sagamihara, Kanagawa 228 Japan;Department of Physiology Kitasato University School of Medicine, Sagamihara, Kanagawa 228 Japan;Molecular Biology, Kitasato University School of Medicine, Sagamihara, Kanagawa 228 Japan

关键词: Natriuretic peptide;    Plasma;    RIA;    HPLC;    reverse-phase;    (Brain);   

DOI  :  10.1016/0014-5793(89)80728-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A highly specific and sensitive radioimmunoassay (RIA) for a novel porcine brain natriuretic peptide (BNP) has been established to elucidate whether BNP-like immunoreactivity (LI) is present in human plasma. The antibody used was specific for BNP without any crossreactivities with known human atrial natriuretic peptides (hANP). After extraction of human plasma with Sep-Pak cartridge, this assay allowed for detection of BNP-LI as low as 0.1 fmol/tube. In 12 healthy subjects, the mean concentrations of plasma BNP-LI were 1.5 fmol/ml. Reverse-phase HPLC coupled with BNP RIA revealed that the single major component with BNP-LI, corresponding to porcine BNP(1–26), was apparently distinct from that of α-hANP. These data indicate that a small molecular mass form with BNP-LI circulates in human blood.

【 授权许可】

Unknown