Isolation of high‐molecular‐weight C‐type natriuretic peptide from the heart of a cartilaginous fish (European dogfish, Scyliorhinus canicula)

FEBS Letters

Suzuki, Reiko⁴  Takahashi, Akiyoshi²  Takei, Yoshio³  Hazon, Neil¹ 

[1] Gatty Marine Laboratory, Department of Biology and Preclinical Medicine, University of St. Andrews, St. Andrews KY16 8LB, UK;Department of Molecular Biology, Kitasata University School of Medicine, Sagamthara, Kanagawa 228, Japan;Department of Physiology, Kitasata University School of Medicine, Sagamthara, Kanagawa 228, Japan;Biotechnology Section, Biotechnology and Medical Business Dept., Sumitamo Metal Industries, Ltd., Sagamihara, Kamagawa 229, Japan

关键词: Natriuretic peptide;    Cartilaginous fish;    Amino acid sequence;    Sequence homology;    Heart;    Scyliorhinus canicula;    ANP;    A-type natriuretic peptide;    Asp-N;    endoprotease Asp-N;    BNP;    B-type natriuretic peptide;    CM;    carboxymethylated;    CNP;    C-type natriuretic peptide;    HPLC;    high-performance liquid chromatography;    LE;    lysyl endopeptidase;    NP;    natriuretic peptide;    TFA;    trifluoroacetic acid;   

DOI  :  10.1016/0014-5793(91)80505-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A high-molecular-weight form of C-type natriuretic peptide (CNP) was isolated from both cardiac atria and ventrieles of European dogfish, Scyliorhinus canicula, and its primary structure was determined. The peptide consists of 115 amino acid residues, in which the C-terminal 22 residues show high homology to CNPs identified to date. This is the first direct evidence for the presence of natriuretic peptide in the cartilaginous fish, and for the presence of CNP in an organ other than the brain.

【 授权许可】

Unknown