Distribution and characterization of immunoreactive adrenomedullin in human tissue and plasma

FEBS Letters

Ichiki, Yoshinari¹  Eto, Tanenao¹  Kangawa, Kenji²  Kawamoto, Mari¹  Kitamura, Kazuo¹  Matsuo, Hisayuki² 

[1] First Department of Internal Medicine, Miyazaki Medical College, Kihara, Kiyotake, Miyazaki 889-16, Japan;National Cardiovascular Research Institute, Suita, Osaka 565, Japan

关键词: Adrenomedullin;    Specific radioimmunoassay;    Distribution;    Characterization;    AM;    adrenomedullin;    RIA;    radioimmunoassay;    HPLC;    high-performance liquid chromatography;    ir-;    immunoreactive;    PC;    pheochromocytoma;    TFA;    trifluoroacetic acid;    BSA;    bovine serum albumin;    CGRP;    calcitonin gene-related peptide;    NPY;    neuropeptide Y;   

DOI  :  10.1016/0014-5793(94)80106-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A specific and sensitive radioimmunoassay for human adrenomedullin has been developed and distribution and characterization of immunoreactive adrenomedullin in human tissue were investigated. The radioimmunoassay specifically recognizes its carboxyterminal region and half maximal inhibition of binding of radioiodinated adrenomedullin(40–52)NH₂ was observed at 11 . Immunoreactive adrenomedullin was abundant in adrenal medulla (47.7 ± 26.1 , mean ± S.D.) and was ubiquitously found in all tissue examined. The mean plasma concentration of adrenomedullin in three normal individuals was 17.2 ± 6.4 (mean ± S.D.). By analysis with reverse-phase high-performance liquid chromatography coupled with the radioimmunoassay, most immunoreactive adrenomedullin in the adrenal medulla, atrium and lung was found to be adrenomedullin(1–52)NH₂.

【 授权许可】

Unknown