Distribution and characterization of immunoreactive rat adrenomedullin in tissue and plasma

FEBS Letters

Sakata, Junichiro¹  Eto, Tanenao¹  Iehiki, Yoshinari¹  Kangawa, Kenji²  Nishizono, Masatoshi¹  Shimokubo, Toru¹  Kitamura, Kazuo¹  Matsuo, Hisayuki² 

[1] First Department of Internal Medicine, Miyazaki Medical College, Kihara Kiyotake, Miyazaki 889-16, Japan;National Cardiovascular Center Research Institute, Fujishirodai, Suita, Osaka 565, Japan

关键词: Adrenomedullin (rat);    Radioimmunoassay;    Distribution;    Characterization;    Plasma;    High-performance liquid;    chromatography;   

DOI  :  10.1016/0014-5793(94)00928-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Adrenomedullin is a new bioactive peptide recently isolated from pheochromocytoma. We report on the rat adrenomedullin distribution and molecular forms in various tissues and plasma. Using a sensitive radioimmunoassay system for rat adrenomedullin, high concentrations of immunoreactive rat adrenomedullin were detected in adrenal gland, lung and cardiac atrium. In lung and atrium, the immunoreactivity concentration in rat was about 6–10 times higher than that in human. The mean plasma concentration of immunoreactive rat adrenomedullin was 3.60 ±0.34 fmol/ml (mean ± S.D.). Analysis in adrenal gland, lung and atrium with reverse-phase and gel-filtration high-performance liquid chromatography showed that most immunoreactive rat adrenomedullin emerged as a single peak at a position exactly identical to that of the authentic rat adrenomedullin peptide, synthesized according to the sequence predicted from the cDNA.

【 授权许可】

Unknown