Radioimmunoassay for circulating human guanylin

FEBS Letters

Adermann, Knut¹  Forssmann, Wolf-Georg¹  Kuhn, Michaela¹  Kulaksiz, Hasan¹  Rechkemmer, Gerhurd¹ 

[1] Lower Saxony Institute for Peptide Research, Feodor-Lynen Str. 31, D-30625 Hannover, Germany

关键词: Guanylin;    Radioimmunoassay;    Human plasma;    Urine;    Blood hemofiltrate;    Dialysis;   

DOI  :  10.1016/0014-5793(94)80460-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A highly specific and sensitive radioimmunoassay for circulating human guanylin (guanylin-22–115) has been developed. Antibodies were raised against the amino-terminus (positions 4–16) of the peptide. Western blot analysis confirmed that the antibody selected for radioimmunoassay recognizes circulating high molecular weight (10.3 kDa) guanylin. Extraction and purification of guanylin from blood hemofiltrate and from blood plasma showed that circulating guanylin is detectable in corresponding amounts by the radioimmunoassay and by a specific bioassay. In 30 healthy subjects, the mean plasma concentration of immunoreactive (IR) guanylin was 42 ± 3 . In 22 patients with chronic renal insufficiency, the concentrations of IR-guanylin were significantly enhanced (1,074 ± 24 ), indicating that kidneys metabolize and/or eliminate the circulating hormone.

【 授权许可】

Unknown