Uroguanylin gene expression in the alimentary tract and extra‐gastrointestinal tissues

FEBS Letters

Matsukura, Shigeru¹  Nakazato, Masamitsu¹  Kangawa, Kenji²  Miyazato, Mikiya²  Matsuo, Hisayuki² 

[1] Third Department of Medicine, Miyazaki Medical College, Kiyotake, Miyazaki 889-16, Japan;National Cardiovascular Center Research Institute, Suita, Osaka 565, Japan

关键词: Uroguanylin;    Guanylin;    Guanylate cyclase C;    cDNA cloning;   

DOI  :  10.1016/S0014-5793(96)01235-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Uroguanylin, a member of the guanylin peptide family, is a novel peptide regulator for intestinal salt and water transport. A cDNA encoding a precursor for rat uroguanylin was cloned from a rat jejunum cDNA library and sequenced. The precursor was 106 amino acids long and included a 21 residue putative signal peptide at the N-terminus. Rat uroguanylin consisted of 15 amino acids similar to, but distinct from human uroguanylin; the C-terminal leucine residue was deleted and 3 residues were substituted compared to those in the human peptide. Synthetic rat uroguanylin-15 dose-dependently increased the cyclic GMP level in cultured T84 cells. RNA blot analysis showed that rat uroguanylin mRNA is expressed not only in the gastrointestinal tract but also in the lung, pancreas and kidney. Evidence for uroguanylin expression in extra-gastrointestinal tissues indicates the possible existence of a novel system for water and electrolyte homeostasis, and a more global effect of uroguanylin on epithelial cell function.

【 授权许可】

Unknown