Topological isomers of human uroguanylin: interconversion between biologically active and inactive isomers

FEBS Letters

Tanabe, Ryosuke²  Chino, Naoyoshi⁴  Nakazato, Masamitsu³  Kobayashi, Yuji²  Kangawa, Kenji¹  Kimura, Terutoshi⁴  Kitani, Tetsuya⁴  Yoshida, Takuya²  Kubo, Shigeru⁴ 

[1] National Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka 565, Japan;Faculty of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565, Japan;Third Department of Internal Medicine, Miyazaki Medical College, Miyazaki 889-16, Japan;Peptide Institute, Inc., Protein Research Foundation, 4-1-2 Ina, Minoh, Osaka 562, Japan

关键词: Uroguanylin;    Guanylin;    Topological isomer;    Interconversion;    HPLC analysis;    Biological activity;    ST;    heat-stable enterotoxin;    cGMP;    cyclic 3′;    5′-guanosine monophosphate;    RP-HPLC;    reversed-phase high performance liquid chromatography;    CD;    circular dichroism;    NMR;    nuclear magnetic resonance;    NOE;    nuclear Overhauser effect;    NOESY;    NOE spectroscopy;    MD;    molecular dynamics;    RMSD;    root mean square deviation;    GdnHCl;    guanidine hydrochloride;    NEM;    N-ethylmaleimide;    TFA;    trifluoroacetic acid;    DMSO;    dimethyl sulfoxide;   

DOI  :  10.1016/S0014-5793(97)01527-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The solution structures of the two compounds of human uroguanylin (I and II), which were generated during disulfide bond forming reaction, were found to be topological isomers by ¹H-nuclear magnetic resonance spectroscopy. These isomers are interconvertible in aqueous media at rates which vary with the pH and temperature of the solution. Because compound I is active in the cGMP producing assay, but compound II is not, this interconversion may be useful for evaluating the activity of human uroguanylin both in vivo and in vitro.

【 授权许可】

Unknown