Immunoreactive adrenomedullin in human plasma

FEBS Letters

Sakakibara, Shumpei²  Ichiki, Yoshinari³  Emura, Junji²  Eto, Tanenao³  Kangawa, Kenji¹  Tanaka, Miho³  Kawamoto, Mari³  Kitamura, Kazuo³  Matsuo, Hisayuki¹ 

[1] National Cardiovascular Center Research Institute, Fujishirodai, Suita, Osaka, Japan;Peptide Institute, Inc. Minoh, Osaka, Japan;First Department of Internal Medicine, Miyazaki Medical College, 5200, Kihara, Kiyotake, Miyazaki Co., Miyazaki, 889-16, Japan

关键词: Adrenomedullin;    Radioimmunoassay;    High-performance liquid chromatography;    Human plasma;   

DOI  :  10.1016/0014-5793(94)80474-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A specific and sensitive radioimmunoassay for adrenomedullin has been developed. Half-maximal inhibition of binding of radioiodinated adrenomedullin was observed at 4 tool/tube. The radioimmunoassay recognized the entire adrenomedullin molecule and has little crossreactivity with adrenomedullin fragment peptides. Adrenomedullin-like immunoreactivity was found to circulate in human plasma at considerable concentration (3.3 ± 0.39 ). The immunoreactivity of adrenomedullin was eluted at almost the same position as synthetic adrenomedullin on gel-filtration chromatography and reverse-phase high-performance liquid chromatography, suggesting that circulating adrenomedullin recognized by the present radioimmunoassay is identical or very similar to authentic adrenomedullin. Plasma immunoreactive adrenomedullin significantly increased in patients with hypertension, with a progressive rise proportionate to disease severity.

【 授权许可】

Unknown