FEBS Letters | |
Immunoreactive adrenomedullin in human plasma | |
Sakakibara, Shumpei2  Ichiki, Yoshinari3  Emura, Junji2  Eto, Tanenao3  Kangawa, Kenji1  Tanaka, Miho3  Kawamoto, Mari3  Kitamura, Kazuo3  Matsuo, Hisayuki1  | |
[1] National Cardiovascular Center Research Institute, Fujishirodai, Suita, Osaka, Japan;Peptide Institute, Inc. Minoh, Osaka, Japan;First Department of Internal Medicine, Miyazaki Medical College, 5200, Kihara, Kiyotake, Miyazaki Co., Miyazaki, 889-16, Japan | |
关键词: Adrenomedullin; Radioimmunoassay; High-performance liquid chromatography; Human plasma; | |
DOI : 10.1016/0014-5793(94)80474-5 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
A specific and sensitive radioimmunoassay for adrenomedullin has been developed. Half-maximal inhibition of binding of radioiodinated adrenomedullin was observed at 4 tool/tube. The radioimmunoassay recognized the entire adrenomedullin molecule and has little crossreactivity with adrenomedullin fragment peptides. Adrenomedullin-like immunoreactivity was found to circulate in human plasma at considerable concentration (3.3 ± 0.39 ). The immunoreactivity of adrenomedullin was eluted at almost the same position as synthetic adrenomedullin on gel-filtration chromatography and reverse-phase high-performance liquid chromatography, suggesting that circulating adrenomedullin recognized by the present radioimmunoassay is identical or very similar to authentic adrenomedullin. Plasma immunoreactive adrenomedullin significantly increased in patients with hypertension, with a progressive rise proportionate to disease severity.
【 授权许可】
Unknown
【 预 览 】
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