Atrial natriuretic peptide (ANP) in rat gastrointestinal tract

FEBS Letters

Vuolteenaho, O.²  Nikkilä, L.²  Ruskoaho, H.¹  Leppäluoto, J.²  Vakkuri, O.²  Maksniemi, T.²  Puurunen, J.¹  Kangas, J.²  Arjamaa, O.² 

[1] Departments of Pharmacology, University of Oulu, 90220 Oulu, Finland;Departments of Physiology, University of Oulu, 90220 Oulu, Finland

关键词: Natriuretic peptide;    Radioimmunoassay;    (Rat;    Stomach;    Intestine);    ANP;    atrial natriuretic peptide;    BSA;    bovine serum albumin;    HPLC;    high-performance liquid chromatography;    IR;    immunoreactive;    PMSF;    phenylmethylsulphonyl fluoride;    RIA;    radioimmunoassay;    TFA;    trifluoroacetic acid;    V 0;    void volume;   

DOI  :  10.1016/0014-5793(88)81359-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Acid extracts of rat stomach and small intestine contained 8.6 ± 3.7 and 39 ± 15 ng/g of immunoreactive atrial natriuretic peptide (ANP). When studied by gel filtration and reverse-phase high-performance liquid chromatography, the stomach immunoreactivity consisted of multiple components, whereas the small intestine contained mostly proANP and ANP 1–28-like material. These findings indicate that ANP may have a role in the physiology of the gastrointestinal tract, e.g. in the regulation of water and electrolyte absorption.

【 授权许可】

Unknown