Radioimmunoassay for leukotriene E4

FEBS Letters

Hoppe, E.M.³  Hoppe, U.²  Peskar, B.M.¹  Peskar, B.A.² 

[1] Department of Experimental Clinical Medicine, Ruhr-University Bochum, D-4630 Bochum, FRG;Department of Pharmacology and Toxicology Ruhr-University Bochum, D-4630 Bochum, FRG;Department of Organic Chemistry II Ruhr-University Bochum, D-4630 Bochum, FRG

关键词: Leukotriene E4 Leukotriene C4 Radioimmunoassay Dimethyl pimelindiimidate (Rat gastric mucosa) γ-Glutamyltranspeptidase;   

DOI  :  10.1016/0014-5793(86)81524-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A conjugate of leukotriene (LT) E₄ and bovine serum albumin (BSA) was prepared by covalently linking the free amino group of the hapten to the protein using dimethyl pimelindiimidate (DMP) as coupling reagent. Anti-LTE₄ antibodies were raised in rabbits immunized with the conjugate. Binding of[³H]LTE₄ to the antibodies is inhibited by 50% with 0.63 ng LTE₄, while the relative cross-reaction of LTC₄ and LTD₄ is 46.3% and 12.6%, respectively. Using the radioimmunoassay release of sulfidopeptide-LT (SP-LT) from rat gastric mucosa incubated in vitro was determined after quantitative enzymatic conversion of SP-LT to LTE₄. It could be demonstrated that this method is suitable for determination of SP-LT in biological material.

【 授权许可】

Unknown