Analysis of β-cryptoxanthin from yellow pigmented marine bacterium Erythrobacter sp. kj5

4th International Conference on Tropical and Coastal Region Eco Development

生态环境科学;经济学

Setiyono, E.^1 ; Brotosudarmo, T.H.P.^1 ; Pringgenies, D.^2 ; Heriyanto^1^3 ; Prihastyanti, M.N.U.^1 ; Shioi, Y.^1

Ma Chung Research Center for Photosynthetic Pigments (MRCPP), Jl. Villa Puncak Tidar N-01, East Java, Malang

65151, Indonesia^1

Department of Marine Science, Universitas Diponegoro, Jl. Prof. Soedarto Tembalang, Semarang

50275, Indonesia^2

Department of Plant Physiology and Biochemistry, Jagiellonian University, ul. Gronostajowa 7, Krakow

30-387, Poland^3

关键词: Carotenoid;    Erythrobacter;    HPLC;    Marine bacterium;    Retention time;    Spectral properties;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/246/1/012004/pdf
DOI  :  10.1088/1755-1315/246/1/012004

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

The objectives of this study were to isolate and analyze β-cryptoxanthin from yellow pigmented marine bacterium Erythrobacter sp. KJ5. The pigments from Erythrobacter sp. KJ5 were extracted from cells with methanol:acetone (7:3, v/v). β-cryptoxanthin standard was isolated from citrus fruit peel using 100% acetone and purified by high-performance liquid chromatography (HPLC) using a C30 column. The existence of β-cryptoxanthin in Erythrobacter sp. KJ5 was determined based on spectral properties and co-chromatography analyses after adding the standard β-cryptoxanthin. The co-chromatography results showed that peak 5 at retention time 33.32 min sharply spiked without changing maximum wavelengths (λmax)at 453 and 480 nm after adding the standard β-cryptoxanthin. These results likely conclude that peak 5 was β-cryptoxanthin.