Isolation and characterization of marine bacteria from macroalgae Gracilaria salicornia and Gelidium latifolium on agarolitic activity for bioethanol production

International Conference on Biomass: Technology, Application, and Sustainable Development

生物科学;能源学;经济学

Kawaroe, M.^1,2 ; Pratiwi, I.^2 ; Sunudin, A.^2

Surfactant and Bioenergy Research Centre, Bogor Agricultural University, Baranang Siang Campus, Bogor

16143, Indonesia^1

Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, Bogor Agricultural University, Darmaga Campus, Bogor

16680, Indonesia^2

关键词: Bio-ethanol production;    Enzyme characteristics;    Enzyme hydrolysis;    High-content;    Isolation and characterization;    Macro-algae;    Marine bacterium;    Qualitative test;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/65/1/012025/pdf
DOI  :  10.1088/1755-1315/65/1/012025

学科分类：生物科学（综合）

来源: IOP

【 摘 要 】

Gracilaria salicornia and Gelidium latifolium have high content of agar and potential to be use as raw material for bioethanol. In bioethanol production, one of the processes level is enzyme hydrolysis. Various microorganisms, one of which is bacteria, can carry out the enzyme hydrolysis. Bacteria that degrade the cell walls of macroalgae and produce an agarase enzyme called agarolytic bacteria. The purpose of this study was to isolate bacteria from macroalgae G. salicornia and G. latifolium, which has the highest agarase enzyme activities, and to obtain agarase enzyme characteristic for bioethanol production. There are two isolates bacteria resulted from G. salicornia that are N1 and N3 and there are two isolates from G. latifolium that are BSUC2 and BSUC4. The result of agarase enzyme qualitative test showed that isolates bacteria from G. latifolium were greater than G. salicornia. The highest agarolitic index of bacteria from G. salicornia produced by isolate N3 was 2.32 mm and isolate N3 was 2.27 mm. Bacteria from G. latifolium produced by isolate BSUC4 was 4.28 mm and isolate BSUC2 was 4.18 mm, respectively. Agarase enzyme activities from isolates N1 and N3 were optimum working at pH 7 and temperature 30 °C, while from isolates BSUC4 was optimum at pH 7 and temperature 50 °C. This is indicated that the four bacteria are appropriate to hydrolyze macro alga for bioethanol production.