Structural and functional analysis of a lycopene β‐monocyclase gene isolated from a unique marine bacterium that produces myxol

FEBS Letters

Ikenaga, Hiroshi²  Takaichi, Shinichi¹  Teramoto, Maki²  Inomata, Yukie²  Misawa, Norihiko² 

[1] Nippon Medical School, Biological Laboratory, Kosugi-cho, Nakahara, Kawasaki-shi 211-0063, Japan;Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi-shi, Iwate 026-0001, Japan

关键词: Lycopene β-cyclase;    γ-Carotene;    Carotenoid;    Marine bacterium;    Flavobacteriaceae;   

DOI  :  10.1016/S0014-5793(03)00513-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A gene coding for lycopene β-monocyclase, which metabolizes lycopene (ψ,ψ-carotene) to γ-carotene (β,ψ-carotene), was isolated for the first time from a unique marine bacterium strain P99-3 that produces myxol (a γ-carotene derivative). This lycopene β-monocyclase gene (designated crtYm) was included in the gene cluster which contained carotenoid biosynthetic gene (crtI, crtB, crtZ, crtY, and crtA) homologs. CrtYm, the CrtY homolog, metabolized lycopene to γ-carotene, which was confirmed by deletion/expression analysis of the crtYm and by subsequent analysis of the metabolites from lycopene based on the retention times on high-performance liquid chromatography, UV–visible absorption spectra, and mass spectrometry.

【 授权许可】

Unknown