Carotenoids inhibit DNA synthesis in human aortic smooth muscle cells

FEBS Letters

Mitchinson, Malcolm J¹  Albarani, Valentina¹  Hardwick, Simon J¹  Carpenter, Keri L.H¹ 

[1] University of Cambridge, Department of Pathology, Tennis Court Road, Cambridge CB2 1QP, UK

关键词: Carotenoid;    Lycopene;    β-Carotene;    DNA synthesis;    Smooth muscle cell (human aortic);    Atherosclerosis;    ABTS;    2;    2′-azinobis(3-ethylbenzothiolazine-6-sulphonate);    AMVN;    2;    2′-azobis(2;    4-dimethylvaleronitrile);    DMEM;    Dulbecco's modified Eagle medium;    DNA;    deoxyribonucleic acid;    FCS;    foetal calf serum;    HMM;    human monocyte macrophage;    HPLC;    high pressure liquid chromatography;    LDL;    low-density lipoprotein;    MDA;    malondialdehyde;    MTT;    3-(4;    5-dimethylthiazol-2-yl)-2;    5-diphenyltetrazolium bromide;    NO2;    nitrogen dioxide;    PBS;    phosphate-buffered saline;    PKC;    protein kinase C;    SMC;    smooth muscle cells;    TBARS;    thiobarbituric acid-reactive substances;    THF;    tetrahydrofuran;    v/v;    volume/volume;   

DOI  :  10.1016/S0014-5793(99)00252-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Quiescent, serum-starved human aortic smooth muscle cells were restimulated with 20% foetal calf serum in Dulbecco's modified Eagle medium, in the presence and absence of β-carotene, canthaxanthin, zeaxanthin, lycopene, lutein or β-cryptoxanthin, at final concentrations up to 23 μM. Concentration-dependent inhibition of DNA synthesis, measured by [methyl-³H]thymidine incorporation, was observed for the carotenoids, except for canthaxanthin and lutein which had no effect. Lycopene was the most potent of the carotenoids tested. The results suggest that antiproliferative effects of dietary carotenoids might be of significance in vivo.

【 授权许可】

Unknown