Isolation, Characterisation and Antagonistic Activity of Bacteria Symbionts Hardcoral Pavona sp. Isolated from Panjang Island, Jepara Against Infectious Multi-drug Resistant (MDR) Bacteria

2nd International Conference on Tropical and Coastal Region Eco Development 2016

地球科学;生态环境科学

Ayuningrum, D.^1 ; Kristiana, R.^1 ; Asagabaldan, M.A.^1 ; Sabdono, A.^2 ; Radjasa, O.K.^2,3 ; Nuryadi, H.^4 ; Trianto, A.^2

Stud. of Coastal Resources Management, Diponegoro University, Semarang

50241, Indonesia^1

Department of Marine Sciences, Diponegoro University, Semarang

50241, Indonesia^2

Ministry of Research, Technology and Higher Education, Indonesia^3

UPT Laboratorium Terpadu, Diponegoro University, Semarang

50241, Indonesia^4

关键词: Acinetobacters;    Antagonistic activity;    Anti-bacterial activity;    Block methods;    Enterobacter aerogenes;    Indonesians;    Marine bacterium;    Pseudoalteromonas;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/55/1/012029/pdf
DOI  :  10.1088/1755-1315/55/1/012029

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Pavona sp. is highly spread over Indonesian waters including Panjang Island. Several studies showed that bacteria symbionts hardcoral were the big source of antibiotic product, but there was limited research of the bacteria symbionts with hardcoral Pavona sp. In this research bacteria symbionts from hardcoral Pavona sp. had been collected from Panjang Island, Jepara. Marine bacteria symbionts were isolated by serial dillution method, while antibacterial activity was performed by using overlay and agar block method. The total of 2 from 5 isolates were active to MDR bacteria such as Enterobacter aerogenes and Acinetobacter baumanii, the code were PHC 44/04 and PHC 44/05. Then both of them were identified by morphological and molecular DNA characterization using 16 S rRNA gene sequence. The result of 16 S rRNA identification shows PHC 44/04 has 99% similarities with Virgibacillus salarius strain sa-Vb 1, while PHC 44/05 shows 99% similarities with Pseudoalteromonas flavipulchra strain NCIMB 2033.