Draft genome sequence of a lipopeptide-producing Bacillus amyloliquefaciens strain isolated from wheat field soil with antagonistic activity against azole-resistant Fusarium graminearum

Journal of Global Antimicrobial Resistance

Jianrong Shi¹  Guizhen Ma²  Gang Wang³  Jianhong Xu³  Yuting Wang⁴ 

[1] College of Life Science, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China;School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, People's Republic of China;Jiangsu Key Laboratory for Food Quality and Safety, State Key Laboratory Cultivation Base, Ministry of Science and Technology/Key Laboratory for Agro-product Safety Risk Evaluation (Nanjing), Ministry of Agriculture and Rural Affairs/Collaborative Innovation Center for Modern Grain Circulation and Safety/Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, People's Republic of China;School of Environmental and Chemical Engineering, Jiangsu Ocean University, Lianyungang, 222005, People's Republic of China;

关键词: Bacillus amyloliquefaciens;    Genome sequence;    Antagonistic activity;    Lipopeptide;    Drug-resistant fungus;   

DOI  :  

来源: DOAJ

【 摘 要 】

ABSTRACT:Objectives: Lipopeptides have been revealed as good potential biocontrol agents against various pathogenic microbes. In the present work, we report the draft genome sequence of a lipopeptide-producing strain of Bacillus amyloliquefaciens (7D3) that showed good antifungal activity against the azole-resistant pathogenic fungus Fusarium graminearum. Methods: Whole-genome sequencing of strain 7D3 was performed on an Illumina MiSeq 300 platform. Raw data were cleaned using Trim Galore v.0.4.0 and were checked for quality using FastQC. De novo assembly was performed using the SOAPdenovo2 package. Genes responsible for the biosynthesis of secondary metabolites were identified using antiSMASH. Results: Bacillus amyloliquefaciens 7D3 genome assembly resulted in a total genome size of 3 913 220 bp with a G+C content of 46.13%. There were 3998 predicted genes with 72 tRNAs and 9 rRNAs. A total of ten gene clusters were found to be related to secondary metabolite biosynthesis, of which five were identified as lipopeptide synthesis clusters. Conclusion: This study presents the genome sequence of B. amyloliquefaciens 7D3, which exhibited intense antagonistic activity against azole-resistant fungi. The whole genome sequence will help in the search for novel antifungal peptides against drug-resistant pathogens.

【 授权许可】

Unknown